Cross Talk Mechanism among EMT, ROS, and Histone Acetylation in Phorbol Ester-Treated Human Breast Cancer MCF-7 Cells

Figure 4

Involvement of intracellular ROS in TPA-elicited EMT processes in MCF-7 cells. MCF-7 cells were pretreated with 20 μM DPI for 1 h and were then treated with 1 nM TPA for 30 min (a), 1 h (b), 6 h (c), or 24 h (d). Intracellular ROS accumulation (a), Slug mRNA expression (b), acetylated histone H3 levels (c), and cell migration (d) were determined. Scale bars show 200 μm. Values (b) are the means of fold changes from vehicle-treated cells (). RT-PCR data were normalized using β-actin levels (, versus vehicle, versus TPA-treated cells).

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