The Protective Effects of Trypsin Inhibitor on Hepatic Ischemia-Reperfusion Injury and Liver Graft Survival

Figure 1

UTI supplementation to the perfusion and preservative LR solutions significantly inhibited liver enzyme release. UTI was added to LR solution at concentration of 10–10000 U/mL; the livers were perfused with 5 mL LR solution with or without UTI supplementation and were then procured and stored in a sterile container containing 2 mL (equal mouse total body blood volume) perfusion solution for 6 hours. The samples were pooled from 3 livers in each group; then, the ALT, AST, and LDH enzyme levels were measured. UTI concentration of 1000 U/mL showed maximal effects. Compared to LR control, . The results are representative of 3 separate experiments.