The Protective Effects of Trypsin Inhibitor on Hepatic Ischemia-Reperfusion Injury and Liver Graft Survival

Figure 2

(a) Liver graft survival from extended cold-preserved donors. Syngeneic orthotopic liver transplantation was performed between inbred C57BL/6 strain mice. The liver donors were preserved in vitro in cold LR solution with or without UTI addition at 1000 U/mL for 1 hour. Liver graft survival was followed posttransplantation. Mean survival time was days in the UTI-treated group () versus days in control (), . (b) Liver histology examination was performed by H&E staining at 1, 6, and 18 hours posttransplantation, respectively (200x). UTI alleviated the degree of liver injury and preserved the cell morphology of the extended-time cold-preserved grafts significantly. Increased hepatocyte swelling, increased cytoplasmic vacuolization, nuclear pyknosis, sinusoidal dilatation, and focal necrosis in the LR-only group with maximal injury at 6 h were observed. (c) Liver pathology scores were evaluated from the H&E sections of (b). The data are from 3 samples in each group and 3 separate experiments.

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