Inhibition of Receptor Interacting Protein Kinases Attenuates Cardiomyocyte Hypertrophy Induced by Palmitic Acid

Figure 1

PA-induced hypertrophy in cardiomyocytes. (a) H9c2 cells were stained by Oil Red O dye. (1) Control (without any treatment). (2) Stimulation with PA (200 μM). (3) Pretreatment with Nec-1 (10 nM), . The result of Oil Red O⁺ (%population) indicated that lipid accumulation was induced in PA stimulation group in H9c2 cells, and the PA-induced intracellular accumulation of neutral lipids in H9c2 cells decreased in PA + Nec-1 group. (b) Gene expression of ANP, BNP, α-MHC, and β-MHC in H9c2 cells was induced by PA, but not OA, . (c) Gene expression of ANP and BNP was upregulated in NCMs, . (d) Fluorescence microscopy observed the increased H9c2 cells surface area of F-actin staining in PA group, which was suppressed by Nec-1 (10 nM), according to the semiquantitative results by ImageJ software, . The red fluorescence indicated cytoskeleton stained by rhodamine phalloidin and the blue fluorescence indicated the cell nucleus stained by DAPI. Data in (a), (b), (c), and (d) are expressed as mean ± SD, indicates compared to control treatment, and indicates compared to PA treatment.

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