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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 1480463, 7 pages
http://dx.doi.org/10.1155/2016/1480463
Research Article

Quantitative Assessment of the Effects of Oxidants on Antigen-Antibody Binding In Vitro

1College of Medical Laboratory, Dalian Medical University, Dalian 116044, China
2Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China

Received 26 December 2015; Revised 4 April 2016; Accepted 28 April 2016

Academic Editor: Domenico D’Arca

Copyright © 2016 Shuang Han et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. We quantitatively assessed the influence of oxidants on antigen-antibody-binding activity. Methods. We used several immunological detection methods, including precipitation reactions, agglutination reactions, and enzyme immunoassays, to determine antibody activity. The oxidation-reduction potential was measured in order to determine total serum antioxidant capacity. Results. Certain concentrations of oxidants resulted in significant inhibition of antibody activity but had little influence on total serum antioxidant capacity. Conclusions. Oxidants had a significant influence on interactions between antigen and antibody, but minimal effect on the peptide of the antibody molecule.