AMP-Activated Protein Kinase Attenuates High Salt-Induced Activation of Epithelial Sodium Channels (ENaC) in Human Umbilical Vein Endothelial Cells

Figure 3

Effect of ENaC blockade on the phosphorylation of eNOS Ser 1177 and production of NO. (a) Representative western blots demonstrating the expression levels of total eNOS, p-eNOS, and β-actin in HUVECs cultured with normal sodium (NS), NS plus 0.5 μM of amiloride (Ami), high sodium (additional 20 mM NaCl treated for 24 h; HS), and HS plus 0.5 μM of amiloride (Ami). (b) Summaries of eNOS activity in response to amiloride in NS or HS group. (c) The images represent the levels of intracellular NO detected using membrane-permeable fluorescent probe, DAF-FM DA, under indicated conditions in HUVECs. (d) Summary of fluorescence results from (c) ( in each group). indicates versus NS group; # represents ; ## indicates versus HS group.

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