AMP-Activated Protein Kinase Attenuates High Salt-Induced Activation of Epithelial Sodium Channels (ENaC) in Human Umbilical Vein Endothelial Cells

Figure 6

High salt-induced reductions in the levels of eNOS phosphorylation and NO production were partially reversed by metformin. (a) Representative western blots demonstrating the expression levels of total eNOS, p-eNOS, and β-actin in HUVECs cultured with NS, NS plus 2 mM metformin (Met), HS, and HS plus 2 mM metformin (Met) for 24 h. (b) Summaries of eNOS activity in response to metformin in NS or HS group. (c) The images represent the levels of intracellular NO detected by a membrane-permeable fluorescent probe, DAF-FM DA, under indicated conditions in HUVECs. (d) Summary of fluorescent intensity analyzed from the experiments shown in (c) ( in each group). and , respectively, indicate and versus NS group; # and ##, respectively, represent and versus HS group.

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