Effect of High-Intensity Training in Normobaric Hypoxia on Thoroughbred Skeletal Muscle

Table 1

Real-time reverse transcriptional-PCR (RT-PCR) primer sequences.

	Forward sequence	Reverse sequence
GAPDH	CAAGGCTGTGGGCAAGGT	GGAAGGCCATGCCAGTGA
Pax7	CATCGGCGGCAGCAA	TCCTCGATCTTTTTCTCCACATC
MyoD	ACGGCTCTCTCTGCAACTTTG	GAGTCGAAACACGGGTCATCA
Myogenin	TCACGGCTGACCCTACAGATG	GGTGATGCTGTCCACAATGG
HIF-1α	AAGTGCGAGCACGATTACAGTAT	GACGGTAGGAAGAGCAGGTTCTT
VEGF-A	CCCACTGCGGAGTTCAACAT	TTGGCTTTGGTGAGGTTTGAT
KDR	TTTTGGCATCACGGAAGTGTAT	TTCTCCGACAAGAGGATATTTCG
PGC-1α	TCCGTGTCACCACCCAAAT	TGAACGAGAGCGCATCCTT
ANGPT1	GCAAATGTGCCCTCATGCT	CAGATTGGATGGGCCACAAG
HGF	GGTACGCTACGAAGTCTGTGACA	CCCATTGCAGGTCATGCAT
FGF-2	TGCGAACCGTTATCTTGCTATG	CACTCGTCCGTAACACATTTAGAAG
IGF-1	TGTCCTCCTCACATCTCTTCTACCT	CGTGGCAGAGCTGGTGAA
IL-6	AACAACTCACCTCATCCTTCGAA	CGAACAGCTCTCAGGCTGAAC

GAPDH: glyceraldehyde-3-phosphate dehydrogenase; Pax7: paired box transcription factor-7; MyoD: myogenic determination factor; HIF-1α: hypoxia inducible factor-1α; VEGF-A: vascular endothelial growth factor-A; KDR: vascular endothelial growth factor receptor-2; PGC-1α: peroxisome proliferator-activated receptor γ coactivator-1α; ANGPT1: angiopoietin-1; HGF: hepatocyte growth factor; FGF-2: fibroblast growth factor-2; IGF-1: insulin-like growth factor-1; IL-6: interleukin-6.