Functional Interactions between BK_Caα-Subunit and Annexin A5: Implications in Apoptosis

Figure 1

Serum deprivation-evoked apoptosis results in early localization of ANXA5 to plasma membrane outer leaflet. (a) The total cell lysates of α HEK239 cells cultured for 48 h in either normal (Cont) or serum-deprived (SD) media where prepared and western blotted to quantify the amounts of ANXA5 and β-actin. (b) HEK 293 cells stably expressing the human α subunit where incubated for 48 hours in before being incubated with biotin reagent. Extracts where prepared and normalized for protein content before the cell surface proteins were isolated with avidin-agarose beads. ANXA5 was detected on the blot using a specific antibody.

(a)

(b)