Functional Interactions between BK_Caα-Subunit and Annexin A5: Implications in Apoptosis

Figure 2

α augments the apoptotic response to serum deprivation: a response which is amplified by ANXA5 knockdown. (a) Caspase-3/7 activity in wild type HEK 293 cells was quantified as relative luminescence units at 24 h and 48 h. Apoptosis was induced by serum deprivation at time = 0 h, with and without ANXA5 knockdown using siRNA (shown beneath the bars). (b) As (a), but using α HEK239 cells. Asterisks indicate that, after 48 h, ANXA5 knockdown enhanced the serum-deprived-evoked caspase activation (, ). (c) Immunocytochemistry using an antibody directed against ANXA5 in α expressing HEK 293 cells following treatment with control (left) and ANXA5 (right) siRNA. (d) Time course of caspase-3/7 activity, α HEK 293 cells (± ANXA5 knockdown) incubated in the presence of normal medium (control), or serum-deprived media (8% Mannitol, DMEM), over a 48-hour period.

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