Berberine Attenuates Myocardial Ischemia/Reperfusion Injury by Reducing Oxidative Stress and Inflammation Response: Role of Silent Information Regulator 1

Figure 11

Effects of berberine and SIRT1 siRNA on SIRT1, Ac-Foxo1, caspase-3, Bcl-2, and Bax expression in SIR-injured cardiomyocyte. H9C2 cardiomyocytes were exposed to control siRNA or SIRT1 siRNA and then treated with BBR (50 μmol/L for 8 hr). Next, cells were subjected to simulated ischemia/reperfusion (SIR, 2 hr/4 hr) treatment. Then, SIRT1-related signaling and apoptosis-related protein were measured. (a) Representative blots. (b) SIRT1 expression. (c) Ac-Foxo1 expression. (d) Caspase-3 expression. (e) Bcl-2 expression. (f) Bax expression. (g) Proposed cardioprotective signaling pathway by BBR. The results are expressed as the mean ± SEM, /group. versus control siRNA group, versus SIR + control siRNA group, and versus SIR + BBR + control siRNA group.

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