Berberine Attenuates Myocardial Ischemia/Reperfusion Injury by Reducing Oxidative Stress and Inflammation Response: Role of Silent Information Regulator 1
Effects of berberine and sirtinol treatment on apoptotic index, infarct size, serum LDH, and serum CK level in MI/R-injured heart. Normal SD rats were exposed to BBR treatment (200 mg/kg/d for 2 weeks) in the absence or presence of sirtinol (Stnl, 2 mg/kg/d for 1 week) and then subjected to myocardial ischemia reperfusion (MI/R) operation. Apoptotic index, infarct size, serum lactate dehydrogenase (LDH), and serum creatine kinase (CK) level were measured after 6 hr of reperfusion. (a) Left: representative photomicrographs of in situ detection of apoptotic cardiomyocytes by TUNEL staining. Green fluorescence shows TUNEL-positive nuclei; blue fluorescence shows nuclei of total cardiomyocytes, original magnification ×400. Right: percentage of TUNEL-positive nuclei. (b) Top: representative photographs of heart sections. Blue-stained portion indicates nonischemic, normal region; red-stained portion, ischemic/reperfused but not infarcted region; and negative-stained portion, ischemic/reperfused infarcted region. Bottom: myocardial infarct size expressed as percentage of area-at-risk (AAR). (c) Serum LDH level. (d) Serum CK level. The results are expressed as the mean ± SEM, /group. versus Sham group, versus MI/R + V group, and versus MI/R + BBR group.
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