Polydatin Protecting Kidneys against Hemorrhagic Shock-Induced Mitochondrial Dysfunction via SIRT1 Activation and p53 Deacetylation

Figure 1

Effect of polydatin (PD) administration on the protein expression and activity of SIRT1 in the renal cortex after HS/R and in HK-2 cells after H/R, respectively. (a) Immunohistochemistry of SIRT1; (b) expression of SIRT1 protein (); (c) SIRT1 activity in the kidney cortex (); (d) expression of SIRT1 protein determined using western blotting (); (e-f) SIRT1 immunocytochemistry using confocal microscopy (original magnification, ×630); (g) determination of SIRT1 activity (). , compared with the value of the vehicle group; , compared with the value of the vehicle group; , compared with the value of the PD group; compared with the value of the PD/Ex527 group. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HS/R, hemorrhagic shock and reperfusion; H/R, hypoxia/reoxygenation; SIRT1, silent information regulator 1; HK-2, human kidney-2.

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