Polydatin Protecting Kidneys against Hemorrhagic Shock-Induced Mitochondrial Dysfunction via SIRT1 Activation and p53 Deacetylation

Figure 4

Effect of polydatin (PD) administration on mitochondrial apoptosis pathway-related protein expression after hypoxia and reoxygenation. (a-b) Apoptosis measured using an annexin V and propidium iodide kit (apoptosis was reflected by the percentage of annexin V positive cells); (c) western blotting of expression of Bax, Bcl-2, cytoplasmic cytochrome C (cyto-cytC), and mitochondrial cytochrome C (mito-cytC) in HK-2 cells; (d–f) quantification of the band intensity of Bax, Bcl-2, and cyto/mito ratio of cytochrome C. The result is a representative band of three independent experiments. compared with the value of the vehicle group; compared with the value of the vehicle group; compared with the value of the PD group; compared with the value of the PD/Ex527 group. HK-2, human kidney-2.

(a)

(b)

(c)

(d)

(e)

(f)