Validation of a Reversed-Phase High Performance Liquid Chromatography Method for the Simultaneous Analysis of Cysteine and Reduced Glutathione in Mouse Organs

Figure 1

HPLC chromatograms of standards of Cys and GSH (20 μM) (a) and of an extract of mouse spleen (b). The exact concentrations of standard solutions of Cys and GSH were obtained through spectrophotometer readings at 412 nm as described in Section 2.

(a)

(b)