Validation of a Reversed-Phase High Performance Liquid Chromatography Method for the Simultaneous Analysis of Cysteine and Reduced Glutathione in Mouse Organs

Table 1

Stability of Cys and GSH in mouse organs.

Sample	−80°C; 3 months	RT; 4 h	4°C; 8 h	Three; freeze-thaw cycles
Cys
Spleen	101.1 ± 4.2	97.6 ± 3.0	99.3 ± 2.9	98.7 ± 1.0
Lymph nodes	97.8 ± 4.6	98.7 ± 7.8	99.8 ± 1.9	98.4 ± 3.9
Pancreas	97.6 ± 2.2	103.0 ± 3.2	96.9 ± 1.8	98.9 ± 1.4
Brain	99.1 ± 2.3	98.4 ± 1.4	99.0 ± 5.1	98.5 ± 3.9
GSH
Spleen	99.9 ± 2.4	97.7 ± 1.7	98.9 ± 2.9	99.2 ± 1.9
Lymph nodes	98.9 ± 1.5	97.9 ± 2.3	99.1 ± 6.9	100.1 ± 1.9
Pancreas	99.3 ± 4.0	97.9 ± 2.0	98.0 ± 3.5	98.4 ± 4.1
Brain	99.6 ± 2.4	99.9 ± 7.5	99.1 ± 7.3	98.7 ± 1.4