Hypochlorite-Modified Albumin Upregulates ICAM-1 Expression via a MAPK–NF-κB Signaling Cascade: Protective Effects of Apocynin

Figure 6

Effects of apocynin on signal transduction underlying HOCl-alb-induced ICAM-1 expression in HUVECs. (a) HUVECs were preincubated with the indicated concentrations of apocynin for 1 h and stimulated with 200 mg/L HOCl-alb for 12 h. ICAM-1 protein expression was analyzed with western blotting. (b) HUVECs were preincubated with 500 μM apocynin for 1 h and then stimulated with 200 mg/L HOCl-alb for 15 min. The activation of ERK1/2 and were determined with western blotting. (c) HUVECs were incubated with 500 μM apocynin for 1 h and stimulated with 200 mg/L HOCl-alb for 30 min. Western blotting was used to detect the nuclear p65 and cytoplasmic p65. Data are expressed as the means ± SD of three independent experiments. ANOVA, in (a), (b), and (c). versus control; versus the HOCl-alb group.

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