Diabetes-Induced Oxidative Stress in Endothelial Progenitor Cells May Be Sustained by a Positive Feedback Loop Involving High Mobility Group Box-1

Figure 2

Expression of HMGB-1 in late EPCs and cell supernatants induced by AGEs at different concentrations. (a) Late EPCs were shown to endocytose Dil-acLDL and bind to lectin. Late EPCs showed a typical “cobblestone” morphology in phase-contrast inverted microscopy after approximately 3 weeks of culture and were positive for expression of CD34, KDR, and CXCR4. (b) Effect of AGEs on HMGB-1 expression in late EPCs. (c) HMGB-1 protein expression in cell culture supernatants of late EPCs exposed to AGEs. Scale bar = 20 μm. versus control group, . BF: bright field; BSA: bovine serum albumin.

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