Diabetes-Induced Oxidative Stress in Endothelial Progenitor Cells May Be Sustained by a Positive Feedback Loop Involving High Mobility Group Box-1

Figure 3

Inhibition of oxidative stress by NAC attenuated HMGB-1 expression in late EPCs. (a) AGE-induced downregulation of sirt1 was partly normalized by resveratrol. (b) Inhibition of sirt1 by Ex527 decreased p66shc expression in AGE-stimulated late EPCs. (c) DCFH-DA staining showing that pretreatment with NAC inhibited ROS production in late EPCs. (d) NAC suppressed HMGB-1 expression in AGE-stimulated late EPCs. between the two groups and between the two groups. NS: no difference between the two groups, . Scale bar = 25 μm.

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