Green Tea Polyphenols Attenuated Glutamate Excitotoxicity via Antioxidative and Antiapoptotic Pathway in the Primary Cultured Cortical Neurons

Figure 4

Involvement of Bcl-2 in the neuroprotection of TP. (a) Representative Western blot analysis of Bcl-2 protein in primary cultured cortical neurons after various treatments. Actin served as internal control. (b) Summary of the optical density of Bcl-2 normalized to actin. Results show that glutamate induced decrease of Bcl-2 was rescued by treatment of TP in the neurons. At least three independent experiments were performed. Data were from all the experiments combined. /group. One-way analysis of variance (ANOVA) followed by Tukey post hoc multiple comparisons tests, . compared to the control group and compared to the Glu group. ((c), (d)) Bar graphs show that TP inhibited glutamate induced neurotoxicity in SiScr treated cortical neurons but not in SiBcl-2 treated neurons as tested by MTT assay. Two independent experiments were performed. Data shown represent one experiment. /group. One-way analysis of variance (ANOVA) followed by Tukey post hoc multiple comparisons tests, for (c); for (d).

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