Green Tea Polyphenols Attenuated Glutamate Excitotoxicity via Antioxidative and Antiapoptotic Pathway in the Primary Cultured Cortical Neurons

Figure 5

Influence of TP on glutamate induced dysregulation of Bax and caspase-3 (Csp-3). (a) Representative Western blot analysis of Bax expression in primary cultured cortical neurons after various treatments. Actin served as internal control. (b) Summary of the optical density of Bax normalized to actin. Results show that TP inhibited glutamate induced upregulation of Bax in the neurons. (c) Representative Western blot analysis of active casapse-3 in primary cultured cortical neurons after various treatments. Actin served as internal control. (d) Summary of the optical density of active caspase-3 normalized to actin. Results show that TP inhibited glutamate induced activation of caspase-3 in the neurons. At least three independent experiments were performed. Data were from all the experiments combined. /group. One-way analysis of variance (ANOVA) followed by Tukey post hoc multiple comparisons tests, for (b); for (d). or compared to the control group and compared to the Glu group.

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