t-BHQ Provides Protection against Lead Neurotoxicity via Nrf2/HO-1 Pathway
Nrf2/HO-1 pathway mediates the protection of t-BHQ against lead toxicity. (a) and (b) con-siRNA, Nrf2-siRNA, or HO-1-siRNA were transfected into SH-SY5Y cells for 12 h before treatment with t-BHQ. The potein levels of Nrf2 and HO-1 were analyzed by Western blot. (c–f) Cells were transfected with con-siRNA, Nrf2-siRNA, or HO-1-siRNA for 12 h and treated with t-BHQ or DMSO for another 12 h and then exposed to PbAc for 24 h in the presence of t-BHQ or DMSO as indicated. Cell viability (c) and caspase 3/7 activity (d) were measured and analyzed. Western blot analyzed the proapoptotic protein of Bax and antiapoptotic protein of Bcl2 in cells transfected with Nrf2-siRNA (e) or HO-1 siRNA (f) and then treated with t-BHQ (40 μM) and PbAc (25 μM) as indicated above. All data represent the mean ± SEM of the results. and represent significant differences.
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