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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 2153745, 10 pages
http://dx.doi.org/10.1155/2016/2153745
Research Article

Hydrogen Sulfide Protects against Chronic Unpredictable Mild Stress-Induced Oxidative Stress in Hippocampus by Upregulation of BDNF-TrkB Pathway

1Department of Neurology, Nanhua Affiliated Hospital, University of South China, Hengyang, Hunan 421001, China
2Institute of Neuroscience, Medical College, University of South China, Hengyang, Hunan 421001, China
3Department of Pathophysiology, Medical College, University of South China, Hengyang, Hunan 421001, China
4Department of Anatomy, Medical College, University of South China, Hengyang, Hunan 421001, China

Received 5 April 2016; Revised 10 June 2016; Accepted 15 June 2016

Academic Editor: Massimo Collino

Copyright © 2016 Min Hu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chronic unpredictable mild stress (CUMS) induces hippocampal oxidative stress. H2S functions as a neuroprotectant against oxidative stress in brain. We have previously shown the upregulatory effect of H2S on BDNF protein expression in the hippocampus of rats. Therefore, we hypothesized that H2S prevents CUMS-generated oxidative stress by upregulation of BDNF-TrkB pathway. We showed that NaHS (0.03 or 0.1 mmol/kg/day) ameliorates the level of hippocampal oxidative stress, including reduced levels of malondialdehyde (MDA) and 4-hydroxy-2-trans-nonenal (4-HNE), as well as increased level of glutathione (GSH) and activity of superoxide dismutase (SOD) in the hippocampus of CUMS-treated rats. We also found that H2S upregulated the level of BDNF and p-TrkB protein in the hippocampus of CUMS rats. Furthermore, inhibition of BDNF signaling by K252a, an inhibitor of the BDNF receptor TrkB, blocked the antioxidant effects of H2S on CUMS-induced hippocampal oxidative stress. These results reveal the inhibitory role of H2S in CUMS-induced hippocampal oxidative stress, which is through upregulation of BDNF/TrkB pathway.