Thioredoxin-Interacting Protein Mediates NLRP3 Inflammasome Activation Involved in the Susceptibility to Ischemic Acute Kidney Injury in Diabetes

Figure 6

HK-2 cells injury after 4 hours of hypoxia followed by 2 hours of reoxygenation under high glucose stimulation. Effects of TXNIP blockage on cell viability assessed by CCK-8 (a); lactate dehydrogenase (LDH) release (b). The data in (a-b) are means ± SE (). versus NG group; versus NH/R group; versus HH/R-scrambled siRNA group; NG: normal glucose (5.6 mM); HG: high glucose (30 mM). NH/R: hypoxia (4 h)/reoxygenation (2 h) under NG condition; HH/R: hypoxia (4 h)/reoxygenation (2 h) under HG condition. HH/R-RES: HH/R pretreated by RES (50 μM) for 72 h with the high glucose incubation. HH/R-siRNA: TXNIP protein was inhibited by transfection with TXNIP siRNA before HH/R; HH/R-scrambled siRNA: scrambled siRNA used as control before HH/R.

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