Dietary Phenolic Compounds Interfere with the Fate of Hydrogen Peroxide in Human Adipose Tissue but Do Not Directly Inhibit Primary Amine Oxidase Activity

Figure 6

Influence of phenolic compounds and MAO inhibitors or SSAO inhibitors on benzylamine oxidation by hAT. (a) Thirty-minute oxidation of 1 mM [¹⁴C]-benzylamine by homogenates generated amounts of radiolabelled benzaldehyde that were separated by extraction as described in Materials and Methods and set at 100% signal (control), while 0% corresponded to the extractible radioactivity at t0. The reference MAO inhibitors (pargyline) or SSAO (semicarbazide) inhibitors were present, separately or in combination at 1 mM, 15 min before the addition of the radiolabelled substrate. (b) Indicated doses of resveratrol (dark circles), pterostilbene (dark squares), caffeic acid (black triangles), or quercetin (open squares) and pargyline (dark circles) were tested on the oxidation of 1 mM [¹⁴C]-benzylamine in the same conditions as for reference inhibitors. Each point is the mean ± SEM of 8 homogenates.

(a)

(b)