Dietary Phenolic Compounds Interfere with the Fate of Hydrogen Peroxide in Human Adipose Tissue but Do Not Directly Inhibit Primary Amine Oxidase Activity

Figure 9

Effects of phenolic compounds, benzylamine, and insulin on glucose transport in human adipocytes. Freshly isolated adipocytes were incubated for 45 min without (basal) or with insulin 100 nM (ins.) or with benzylamine and phenolic compounds at the indicated doses. Then 2-DG uptake assays were performed on 10 min as detailed in Section 2. (a) Blockade of benzylamine insulin-like stimulation of glucose uptake in human adipocytes by amine oxidase inhibitors. Basal, insulin-stimulated, or benzylamine-stimulated 2-DG uptake was tested without (no addition, open columns) or with pargyline + semicarbazide at 1 mM each (P + S, dark columns). Mean ± SEM of 39 cases. Different from basal alone at . Different from benzylamine alone at . (b) Insulin was used as the agent of reference for maximal transport stimulation, set at 100%. Benzylamine was tested at 1 mM alone (control, open column) or in the presence of 100 μM of the indicated phenolic compounds. Mean ± SEM of 7 observations. Different from benzylamine alone at and .

(a)

(b)