Research Article
Nigella sativa Relieves the Altered Insulin Receptor Signaling in Streptozotocin-Induced Diabetic Rats Fed with a High-Fat Diet
Table 2
PCR primers and programs of PCR cycle.
| | Gene | Forward primer | Reverse primer | PCR program |
| | -actin | 5′-CTGACCGAGCTGGCTAC-3′ | 5′-CCTGCTTGCTGATCCACA-3′ | 94/2/53.4/30/72/30/30 | | ADAM-17 | 5′-TAGCAGATGCTGGTCATGTG-3′ | 5′-TTGCACCACAGGTCAAAAG-3′ | 94/2/50/30/72/30/30 | | PI3K | 5′-TTAAACGCGAAGGCAACGA-3′ | 5′-CAGTCTCCTCCTGCTGTCGAT-3′ | 94/2/53.7/30/72/30/30 | | IGF-1 | 5′-ACTTCTGCGCCAACATCCTCA-3′ | 5′-CCCTTTAGTCCCCGTCACTTCC-3′ | 94/2/57/30/72/30/30 | | IR | 5′-TGACAATGAGGAATGTGGGGAC-3′ | 5′-GGGCAAACTTTCTGACAATGACTG-3′ | 94/2/53.3/30/72/30/30 |
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