Research Article
Modulation of Hydrogen Peroxide-Induced Oxidative Stress in Human Neuronal Cells by Thymoquinone-Rich Fraction and Thymoquinone via Transcriptomic Regulation of Antioxidant and Apoptotic Signaling Genes
Figure 3
Effects of Thymoquinone-rich fraction (TQRF) and Thymoquinone (TQ) on intracellular ROS production. SH-SY5Y cells treated with different concentrations of (a) TQRF, (b) TQ (μg/mL), and (c) 1% TQ in TQRF (ng/mL) prior to H2O2 (250 μM) exposure. The fluorescence intensities for each treatment were recorded versus time up to 3 h. The percentage of ROS production (mean ± SD) relative to control cells in the presence of (d) TQRF/TQ (μg/mL) and (e) TQ (ng/mL). In each panel, mean values labeled with different alphabets are significantly different at .
| (a) TQRF (µg/mL) |
| (b) TQ (µg/mL) |
| (c) TQ (ng/mL) |
| (d) TQRF/TQ (µg/mL) |
| (e) TQ (ng/mL) |