Modulation of Hydrogen Peroxide-Induced Oxidative Stress in Human Neuronal Cells by Thymoquinone-Rich Fraction and Thymoquinone via Transcriptomic Regulation of Antioxidant and Apoptotic Signaling Genes
Table 1
Gene name, accession number, and reverse and forward primer sequences used in GeXP multiplex gene expression analysis.
Gene name
Accession number
Primer sequences with universal tags
Forward
Reverse
Antioxidant genes
SOD 1
NM_000454
AGGTGACACTATAGAATATCATCAATTTCGAGCAGAAGG
GTACGACTCACTATAGGGATGCTTTTTCATGGACCACC
SOD 2
NM_000636
AGGTGACACTATAGAATACATCAAACGTGACTTTGGTTC
GTACGACTCACTATAGGGACTCAGCATAACGATCGTGGTT
Catalase
NM_001752
AGGTGACACTATAGAATAGAAGTGCGGAGATTCAACACT
GTACGACTCACTATAGGGAACACGGATGAACGCTAAGCT
Apoptotic genes
ERK1/2
NM_002745
AGGTGACACTATAGAATAGGAGCAGTATTACGACCCGA
GTACGACTCACTATAGGGAGATGTCTGAGCACGTCCAGT
p53
NM_001126117
AGGTGACACTATAGAATAGGGGAGCAGGGCTCA
GTACGACTCACTATAGGGAAAAATGGCAGGGGAGGG
JNK
NM_139046
AGGTGACACTATAGAATACAGAAGCTCCACCACCAAAGAT
GTACGACTCACTATAGGGAGCCATTGATCACTGCTGCAC
AKT1
NM_001014431
AGGTGACACTATAGAATAGAGGAGATGGACTTCCGGTC
GTACGACTCACTATAGGGAAGGATCTTCATGGCGTAGTAGC
NF-kB
NM_001077493
AGGTGACACTATAGAATAGCGGGCGTCTAAAATTCTG
GTACGACTCACTATAGGGATTCCACGATCACCAGGTAGG
p38
NM_001315
AGGTGACACTATAGAATATTCAGTCTTTGACTCAGATGCC
GTACGACTCACTATAGGGAGTCAGGCTTTTCCACTCATCT
Housekeeping genes
GAPDH
NM_002046
AGGTGACACTATAGAATAAAGGTGAAGGTCGGAGTCAA
GTACGACTCACTATAGGGAGATCTCGCTCCTGGAAGATG
Hyaluronidase
AJ000099
AGGTGACACTATAGAATACAGCAGTTCATGCTGGAGAC
GTACGACTCACTATAGGGACCAGGTAGACAGACGGGAAG
18 sRNA
M10098
AGGTGACACTATAGAATAGGAGTGGAGCCTGCGGCTTAA
GTACGACTCACTATAGGGATAGCATGCCAGAGTCTCGTT
Actb
NM_001101
AGGTGACACTATAGAATAGATCATTGCTCCTCCTGAGC
GTACGACTCACTATAGGGAAAAGCCATGCCAATCTCATC
Kan(r)
—
AGGTGACACTATAGAATAATCATCAGCATTGCATTCGATTCCTGTTTG
GTACGACTCACTATAGGGAATTCCGACTCGTCCAACATC
Based on the Homo sapiens gene sequences adopted from the National Center for Biotechnology Information GenBank Database. Housekeeping genes, Internal control, and Normalization gene.
