Research Article

Melatonin-Mediated Intracellular Insulin during 2-Deoxy-d-glucose Treatment Is Reduced through Autophagy and EDC3 Protein in Insulinoma INS-1E Cells

Figure 4

Expression of Cu/Zn-SOD, Mn-SOD, catalase, Bcl-2, and Bax proteins in the presence or absence of melatonin and/or 2-DG in rat insulinoma INS-1E cells. INS-1E cells were incubated in RPMI 1640 medium supplemented with 2% FBS with/without melatonin (100 μM) and/or 2-DG (5 mM) for 24 hr at 37°C with 5% CO2. Cu/Zn-SOD, Mn-SOD, and catalase proteins were analyzed by Western blot (a, e). The relative amounts of Cu/Zn-SOD (b), Mn-SOD (c), catalase proteins (d), Bcl-2 (f), and Bax proteins (g) were quantified in Section 2. Data represent mean ± SD of three experiments (). , versus 2% FBS; , , and , melatonin versus melatonin and/or 2-DG.