Melatonin-Mediated Intracellular Insulin during 2-Deoxy-d-glucose Treatment Is Reduced through Autophagy and EDC3 Protein in Insulinoma INS-1E Cells
Expression of LC3, insulin, and EDC3 proteins in the presence or absence of melatonin and/or 2-DG in rat insulinoma INS-1E cells. INS-1E cells were incubated in RPMI 1640 medium supplemented with 2% FBS with/without melatonin (100 μM) and/or 2-DG (5 mM) for 24 hr at 37°C with 5% CO2. LC3, Insulin, and EDC3 proteins were analyzed by Western blot (a). The relative amounts of LC3 (b), insulin (c), and EDC3 proteins (d) were quantified in Section 2. Data represent mean ± SD of three experiments (). , versus 2% FBS; , melatonin versus melatonin and/or 2-DG.