Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation

Figure 1

Identification of polyphenols that augment SIRT3 transcription in Caco-2 cells. Polyphenols (10 μM) and their corresponding amount of DMSO were added to Caco-2 cells transduced with SIRT3p-EGFP and changes in EGFP fluorescence were monitored by an IN Cell Analyzer 1000 (a). Dose-dependent effects of polyphenols on the expression of endogenous SIRT3 in Caco-2 cells were assessed by qRT-PCR. Control Caco-2 cells were treated with the same volume of DMSO used for treating with 1, 10, and 50 μM of polyphenols. (b) The results are expressed as mean ± standard deviation. Statistical significance between the respective concentration and its corresponding in the control was determined by one-way ANOVA with Tukey’s post hoc test. Statistical significance was defined as (;  ). C3g, cyanidin 3-glucoside chloride; D3g, delphinidin 3-glucoside chloride; CA, chlorogenic acid.

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