Pomegranate-Derived Polyphenols Reduce Reactive Oxygen Species Production via SIRT3-Mediated SOD2 Activation

Figure 3

SIRT3-dependency of pomegraniin A-induced SOD2 activation. Effects of eucalbanin B (a) and pomegraniin A (b) on SOD2 activity in SIRT1- and SIRT3-silenced Caco-2 cells were evaluated using the SOD Assay Kit-WST. The results are expressed as mean ± standard deviation. Control Caco-2 cells were treated with the same volume of DMSO used for treating with 10 μM of polyphenols. Statistical significance between the respective polyphenol and control was determined by one-way ANOVA with Tukey’s post hoc test. Statistical significance was defined as (; ). SOD2, superoxide dismutase 2.

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