Adiponectin Protects against Glutamate-Induced Excitotoxicity via Activating SIRT1-Dependent PGC-1 Expression in HT22 Hippocampal Neurons

Figure 1

Effects of different concentrations of the APN peptide on HT22 cells. (a) HT22 cells were incubated with the APN peptide at different concentrations (50 μM, 100 μM, 250 μM, or 500 μM) for 24 h. Cell viability is shown in OD. (b) LDH release detection after treatments with indicated concentrations of APN peptide. The results are expressed as fold increase of the control levels. (c) SIRT1 and PGC-1α levels were analyzed by western blotting, and β-actin was used as the control protein. (d) SIRT1 expression level. (e) PGC-1α expression level. The data are presented as the mean ± SD. ; versus the control group; versus the 100 μM APN peptide treatment group; versus the 250 μM APN peptide treatment group; APN, adiponectin; OD, optical density; LDH, lactate dehydrogenase; SIRT1, silent information regulator 1; PGC-1α, peroxisome proliferator-activated receptor gamma coactivator 1-alpha.

(a)

(b)

(c)

(d)

(e)