Caspase-3 Deletion Promotes Necrosis in Atherosclerotic Plaques of ApoE Knockout Mice

Figure 4

Caspase-3 deficiency in macrophages does not trigger a switch to necroptosis. BMDM were isolated from Casp3^+/+ApoE^−/− (Casp3^+/+) and Casp3^−/−ApoE^−/− (Casp3^−/−) mice and treated with cycloheximide (CHX) (0–30 μg/mL) or oxLDL (25–75 μg/mL) in the presence or absence of the necroptosis inhibitor Necrostatin-1 (Nec-1) (30 μmol/L). BMDM treated with 100 ng/mL LPS and 20 μmol/L zVAD-fmk were used as positive control for necroptosis induction. Necrosis was monitored by PI labeling ( independent experiments with 2 counting regions of 150 cells/region in duplicate; NS, not significant; versus Casp3^+/+ and Casp3^−/− without Nec-1; factorial ANOVA with genotype and treatment as category factors; Dunnett post hoc).