Lycopene Ameliorates Transplant Arteriosclerosis in Vascular Allograft Transplantation by Regulating the NO/cGMP Pathways and Rho-Associated Kinases Expression

Figure 3

Expression levels of ICAM-1 and eNOS were determined by immunohistochemical staining and real-time quantitative PCR analysis. ((a1)–(a3) and (b1)–(b3)) Immunohistochemical staining of ICAM-1 and eNOS in grafted arteries (original magnification ×200). ((a4) and (b4)) The mean of IOD/area of ICAM-1 and eNOS immunohistochemical staining in each group. (a4) , allograft group compared with the isograft group; , lycopene group compared with the allograft group. (b4) , . ((a5) and (b5)) Real-time quantitative PCR analysis of ICAM-1 and eNOS mRNA expression in each group. (a5) , . (b5) and . ((a1) and (b1)) Isograft group; ((a2) and (b2)) allograft group; ((a3) and (b3)) lycopene group.