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Oxidative Medicine and Cellular Longevity
Volume 2016 (2016), Article ID 3134919, 11 pages
http://dx.doi.org/10.1155/2016/3134919
Research Article

An Explanation of the Underlying Mechanisms for the In Vitro and In Vivo Antiurolithic Activity of Glechoma longituba

1Department of Urology, Institute of Urology, Gansu Nephro-Urological Clinical Center, Key Laboratory of Urological Diseases in Gansu Province, The Second Hospital of Lanzhou University, Lanzhou, Gansu 730030, China
2Department of Neurosurgery, The Neurosurgery Clinical Medical Center, The Second Hospital of Lanzhou University, Lanzhou, Gansu 730030, China
3Department of Urology, First Affiliated Hospital of Anhui Medical University and Institute of Urology, Anhui Medical University, Hefei, Anhui, China
4First Clinical Medical College of Lanzhou University, Lanzhou, Gansu 730000, China
5Department of General Surgery, Lanzhou University Second Hospital, Lanzhou 730030, China
6Orthopaedics Key Laboratory of Gansu Province, Department of Orthopaedics, The Second Hospital of Lanzhou University, Lanzhou, Gansu, China
7Department of Cardiology, Lanzhou University Second Hospital, Lanzhou 730030, China

Received 19 July 2016; Revised 26 August 2016; Accepted 27 September 2016

Academic Editor: Francisco J. Romero

Copyright © 2016 Qiang Liang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. To use in vitro and in vivo models to evaluate Glechoma longituba extract to provide scientific evidence for this extract’s antiurolithic activity. Materials and Methods. Potassium citrate was used as a positive control group. Oxidative stress (OS) markers and the expression of osteopontin (OPN) and kidney injury molecule-1 (KIM-1) were measured to assess the protective effects of Glechoma longituba. Multiple urolithiasis-related biochemical parameters were evaluated in urine and serum. Kidneys were harvested for histological examination and the assessment of crystal deposits. Results. In vitro and in vivo experiments demonstrated that treatment with Glechoma longituba extract significantly decreased calcium oxalate- (CaOx-) induced OPN expression, KIM-1 expression, and OS compared with the positive control group (). Additionally, in vivo rats that received Glechoma longituba extract exhibited significantly decreased CaOx deposits and pathological alterations () compared with urolithic rats. Significantly lower levels of oxalate, creatinine, and urea and increased citrate levels were observed among rats that received Glechoma longituba () compared with urolithic rats. Conclusion. Glechoma longituba has antiurolithic effects due to its possible combined effects of increasing antioxidant levels, decreasing urinary stone-forming constituents and urolithiasis-related protein expression, and elevating urinary citrate levels.