Renalase as a Novel Biomarker for Evaluating the Severity of Hepatic Ischemia-Reperfusion Injury

Figure 1

Increase of renalase expression in hepatic cells upon H₂O₂ treatment. (a) Relative expression of renalase mRNA evaluated by real-time qPCR in HepG2 cells treated with increasing concentrations of H₂O₂. (b) Relative expression of renalase mRNA evaluated by real-time qPCR in HepG2 cells treated with H₂O₂ for different times. (c) Western blot analysis of renalase protein levels in HepG2 cells treated with 500 μM H₂O₂ for 6 h or 12 h. (d) Densitometric analysis of Western blot of renalase in HepG2 cells treated with 500 μM H₂O₂ for 6 h or 12 h. ; , compared with control group. Data are plotted as the mean ± SD from five independent experiments. Bars indicate the standard deviation of the mean.

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