Renalase as a Novel Biomarker for Evaluating the Severity of Hepatic Ischemia-Reperfusion Injury

Figure 2

Antioxidant-induced decrease of H₂O₂-induced augmentation of the renalase expression in hepatic cells. (a) Relative expression of renalase mRNA evaluated by real-time qPCR in H₂O₂-incubated (12 h) HepG2 cells with or without pretreatment with antioxidants. (b) Western blot analysis of renalase protein levels in H₂O₂-incubated (12 h) HepG2 cells with or without preincubation with antioxidants. (c) Densitometric analysis of Western blot of renalase in H₂O₂-incubated HepG2 cells with or without preincubation with antioxidants. . Data are plotted as the mean ± SD from five independent experiments. Bars indicate the standard deviation of the mean.

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