Renalase as a Novel Biomarker for Evaluating the Severity of Hepatic Ischemia-Reperfusion Injury

Figure 4

Increase of renalase levels in hepatic I/R injury models and its suppression induced by antioxidant preinjection and the corresponding histological changes in liver. (a) The livers of the sham, I/R, and IR+SOD+CAT groups (6 h after reperfusion) were subjected to histological evaluation by H&E staining and IHC of cleaved caspase-3. Scale bar (black) represents 50 μm. (b) Confocal immunofluorescence imaging of renalase in livers of mouse models (sham, I/R injury, and I/R injury with antioxidant preinjection, 6 h after reperfusion). Scale bar (white) represents 30 μm. The relative fluorescent density of each group was analyzed. . Data are plotted as the mean ± SD from five independent experiments. Bars indicate the standard deviation of the mean.

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