Evidence for Detrimental Cross Interactions between Reactive Oxygen and Nitrogen Species in Leber’s Hereditary Optic Neuropathy Cells

Figure 1

Bioenergetic properties of 11778/ND4 mutated PBMCs. (a) Representative cell O₂ consumption measurements. Black line: O₂ concentration trace; red line: O₂ consumption rate. After recording basal respiration, 4 μM antimycin A (AA) was added. (b) Cellular ATP levels measured in the presence of glucose with and without oligomycin. (c) Cellular ATP levels measured in the absence of glucose with and without oligomycin. (d) Fractional ATP expressed as the ratio between and .    was obtained from ΔATP (c) and was measured in the presence of glucose without oligomycin (b). Data (mean ± SEM) were collected in three triplicate experiments on cells derived from the LHON patient and the three healthy controls.

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