Evidence for Detrimental Cross Interactions between Reactive Oxygen and Nitrogen Species in Leber’s Hereditary Optic Neuropathy Cells

Figure 2

Oxidative and nitrosative stress is increased in 11778/ND4 mutated PBMCs. (a) Intracellular ROS levels were measured using the DCFDA dye. (b) The 3-NT content was determined by competitive ELISA and normalized to total protein. (c) Concentration of NO_x (nitrite and nitrate) in the cell supernatant as normalized to total protein. Data (mean ± SEM) collected in three duplicate experiments on cells derived from the LHON patient and the three healthy controls. Values are considered significant when : ; Ns: not significant.

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