Inhibition of HSP90 Promotes Neural Stem Cell Survival from Oxidative Stress through Attenuating NF-κB/p65 Activation

Figure 2

NSCs survival from oxidative stress induced by H₂O₂ treatment. The NSCs were subjected to 100 M H₂O₂ induction for 2 h, following cell starving within the DMEM/F12 medium without B27, EGF, and bFGF. The NSCs treated with the vehicle medium were used as control. (a) The cell viability by MTT assay of the NSCs. (b) The cell morphology of the NSCs. (c) The ROS production of the NSCs probed by DCF-DA. (d) The apoptosis and cell death of the NSCs detected by Annexin V-FITC/PI kit. (e) Quantified positive cell number of the NSCs with Annexin V-FITC/PI staining. was considered to be significantly different between control and H₂O₂ groups.

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