Inhibition of HSP90 Promotes Neural Stem Cell Survival from Oxidative Stress through Attenuating NF-κB/p65 Activation

Figure 4

Effect of Geldanamycin and JSH-23 on H₂O₂ induced NF-B/p65 and HSP90 activation. The pretreatment of 0.5 M GA or 8 M JSH-23 was administrated to NSCs for 1 h, followed by the H₂O₂ induction. (a)–(c) The protein expression of phospho-IKK, total IKK, phospho-IB, total IB, nuclear phospho-p65, and total p65 in the NSCs treated with GA and H₂O₂. (d) The protein expression of HSP90 in the NSCs treated with JSH-23 and H₂O₂. The protein expression in NSCs cultured within the vehicle medium was used as control. and were considered to be significantly different between control and H₂O₂ or between H₂O₂ and GA/JSH-23+H₂O₂ groups. n = 3.

(a)

(b)

(c)

(d)