Oxidative Medicine and Cellular Longevity / 2016 / Article / Fig 2

Research Article

Protective Effect of Peroxisome Proliferator-Activated Receptor α Activation against Cardiac Ischemia-Reperfusion Injury Is Related to Upregulation of Uncoupling Protein-3

Figure 2

WY-14643 increased UCP3 expression in rats. WY-14643 20 mg/kg was administered 4 h before the extraction of heart. (a) Quantitative PCR shows that WY-14643 increased mRNA levels compared with vehicle. (). (b) RT-PCR shows increased UCP3 mRNA expression following WY-14643 administration. (c) Western blot shows increased expression of UCP3 and HO-1, and phosphorylation of Akt and Erk after WY-14643 administration. Band intensities were quantified using an image analyzer and normalized by expression levels of β-actin. (). (d) Representative blots are shown.

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