Protective Effect of Peroxisome Proliferator-Activated Receptor α Activation against Cardiac Ischemia-Reperfusion Injury Is Related to Upregulation of Uncoupling Protein-3

Figure 4

WY-14643 decreased hypoxia-stimulated intracellular ROS production. (a) Cells treated with 50 μM of WY-14643 or vehicle were subjected to 20 h of hypoxia and ROS level was measured by DCF fluorescence. Hypoxia increased ROS generation and WY-14643 attenuated hypoxia-induced increase in ROS. UCP3 siRNA abrogated the effect of WY-14643 on ROS production. versus control. versus hypoxia (). (b) Cells treated with 50 μM of WY-14643 or vehicle were subjected to 20 h of hypoxia and ROS level was measured by lucigenin-amplified chemiluminescence. Hypoxia increased ROS generation and WY-14643 attenuated hypoxia-induced increase in ROS. UCP3 siRNA abrogated the effect of WY-14643 on ROS production (). (c) Cells treated with 50 μM of WY-14643 or vehicle were subjected to 20 h of hypoxia and mitochondrial superoxide was measured by MitoSOX Red fluorescence. Hypoxia increased mitochondrial superoxide generation and WY-14643 attenuated hypoxia-induced increase in mitochondrial superoxide. UCP3 siRNA abrogated the effect of WY-14643 on mitochondrial superoxide production. versus control. versus hypoxia (). Hyp: hypoxia; WY: WY-14643 treatment.

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