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Oxidative Medicine and Cellular Longevity
Volume 2016 (2016), Article ID 3620929, 8 pages
http://dx.doi.org/10.1155/2016/3620929
Research Article

Exercise-Induced Changes in Caveolin-1, Depletion of Mitochondrial Cholesterol, and the Inhibition of Mitochondrial Swelling in Rat Skeletal Muscle but Not in the Liver

1Department of Bioenergetics and Physiology of Exercise, Medical University of Gdansk, 80-210 Gdansk, Poland
2Department of Bioenergetics and Nutrition, Gdansk University of Physical Education and Sport, 80-336 Gdansk, Poland
3Department of Physiotherapy, Gdansk University of Physical Education and Sport, 80-336 Gdansk, Poland
4Department of Physiotherapy, Medical University of Gdansk, 80-210 Gdansk, Poland
5Department of Biochemistry, Gdansk University of Physical Education and Sport, 80-336 Gdansk, Poland
6Department of Medical Chemistry, Medical University of Gdansk, 80-210 Gdansk, Poland
7School of Pharmacy, University of Camerino, 62032 Camerino, Italy

Received 29 July 2015; Revised 22 September 2015; Accepted 11 October 2015

Academic Editor: Rickie Simpson

Copyright © 2016 Damian Jozef Flis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The reduction in cholesterol in mitochondria, observed after exercise, is related to the inhibition of mitochondrial swelling. Caveolin-1 (Cav-1) plays an essential role in the regulation of cellular cholesterol metabolism and is required by various signalling pathways. Therefore, the aim of this study was to investigate the effect of prolonged swimming on the mitochondrial Cav-1 concentration; additionally, we identified the results of these changes as they relate to the induction of changes in the mitochondrial swelling and cholesterol in rat skeletal muscle and liver. Male Wistar rats were divided into a sedentary control group and an exercise group. The exercised rats swam for 3 hours and were burdened with an additional 3% of their body weight. After the cessation of exercise, their quadriceps femoris muscles and livers were immediately removed for experimentation. The exercise protocol caused an increase in the Cav-1 concentration in crude muscle mitochondria; this was related to a reduction in the cholesterol level and an inhibition of mitochondrial swelling. There were no changes in rat livers, with the exception of increased markers of oxidative stress in mitochondria. These data indicate the possible role of Cav-1 in the adaptive change in the rat muscle mitochondria following exercise.