Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation

Figure 2

Proliferation and morphology of ASC. Using resazurin-based assay, the growth kinetics of isolated cells was established. During the seven-day test displayed decreased proliferation potential in comparison to control group (a). Flow cytometry analysis confirmed decreased expression of Ki67 in those cells (b). Morphology of investigated cells was evaluated after day 7. While ASC_CTRL reached high confluence and developed robust net of cytoskeletal projections (lamellipodia and filopodia), ASC_EMS did not form multilayer and were more amorphous in shape with reduced cellular projections (c). Interestingly, there were no differences in the expression of mir-489, which plays crucial role in the differentiation process (d). Results are expressed as mean ± SD. value < 0.001.

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