Macroautophagy and Selective Mitophagy Ameliorate Chondrogenic Differentiation Potential in Adipose Stem Cells of Equine Metabolic Syndrome: New Findings in the Field of Progenitor Cells Differentiation

Table 2

Sequences of primers used in qPCR.

Gene	Primer	Sequence 5′-3′	Amplicon length (bp)	Accession number
MnSOD	F	CAATAAGGACCAGGGACGCC	244	XM_014858346.1
	R	GCTTAATGCACTCGGTGTAACG
Parkin	F	TCCCAGTGGAGGTCGATTCT	218	XM_005608126.2
	R	CCCTCCAGGTGTGTTCGTTT
PGC1α	F	TCTACCTAGGATGCATGG	93	XM_005608845.2
	R	GTGCAAGTAGAAACACTGC
HIF-1-α	F	CTCAAATGCAAGAACCTGCTC	86	XM_014735822.1
	R	TTCCATACCATCTTTTGTCACTG
Vimentin	F	GCAGGATTTCTCTGCCTCTT	352	XM_014852743.1
	R	TATTGCTGCACCAAGTGTGT
FOXO1	F	ATTGAGCGCTTGGACTGTGA	311	XM_014732057.1
	R	CGCTGCCAAGTTTGACGAAA
LC3	F	TTACTGCTTTGCTCTGCCAC	213	XM_005608485.2
	R	AGCTGCTTCTCCCCCTTGT
Beclin	F	GATGCGTTATGCCCAGATGC	147	XM_014729146.1
	R	ATCCAGCGAACACTCTTGGG
LAMP2	F	GCACCCCTGGGAAGTTCTTA	139	XM_014733098.1
	R	TTCGAGGATCTGTGCCAATCA
GAPDH	F	GATGCCCCAATGTTTGTGA	250	NM_001163856.1
	R	AAGCAGGGATGATGTTCTGG
CHOP	F	AGCCAAAATCAGAGCCGGAA	272	XM_014844003.1
	R	GGGGTCAAGAGTGGTGAAGG
PERK	F	GTGACTGCAATGGACCAGGA	283	XM_014852775.1
	R	TCACGTGCTCACGAGGATATT
PINK	F	GCACAATGAGCCAGGAGCTA	298	XM_014737247.1
	R	GGGGTATTCACGCGAAGGTA
PARKIN	F	TCCCAGTGGAGGTCGATTCT	218	XM_014858374.1
	R	CCCTCCAGGTGTGTTCGTTT
FIS	F	GGTGCGAAGCAAGTACAACG	118	XM_001504462.4
	R	GTTGCCCACAGCCAGATAGA
MFN	F	AAGTGGCATTTTTCGGCAGG	217	XM_001495170.5
	R	TCCATATGAAGGGCATGGGC
Decorin	F	GATGCAGCTAGCCTGAGAGG	248	XM_014841263.1
	R	GTGTTGTATCCAGGTGGGCA
Sox-9	F	GAACGCCTTCATGGTGTGGG	225	XM_014736619.1
	R	TTCTTCACCGACTTCCTCCG

Sequences, amplicon length, and accession numbers of the primer sets. MnSOD: mitochondrial superoxide dismutase 2; Parkin: parkin RBR E3 ubiquitin protein ligase (PARK2); PGC1 α: peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; HIF-1-α: hypoxia inducible factor 1, alpha subunit; Bcl-2: B-cell CLL/lymphoma 2; FOXO1: forkhead box O1; LC3: microtubule associated protein 1 light chain 3 beta (MAP1LC3B); Beclin: beclin 1, autophagy related (BECN1); LAMP2: lysosomal-associated membrane protein 2; GADPH: glyceraldehyde-3-phosphate dehydrogenase; CHOP: DNA damage inducible transcript 3 (DDIT3); PERK: eukaryotic translation initiation factor 2-alpha kinase 3; PINK: PTEN-induced putative kinase 1; PARKIN: parkin ligase; FIS: fission, mitochondrial 1; MFN: mitofusin 1 (MFN1); Sox-9: transcription factor SOX-9.