Regular and Moderate Exercise Counteracts the Decline of Antioxidant Protection but Not Methylglyoxal-Dependent Glycative Burden in the Ovary of Reproductively Aging Mice

Figure 6

Ovarian transcriptional expression and enzymatic activity of glyoxalase 1 (GLO1) in mice after regular and moderate treadmill running. GLO1 mRNA levels (a) and specific activity (b) of GLO1 in ovaries of middle-aged CD1 female mice after two- or four-month running programme (E2 or E4, resp.); age-matched sedentary animals (S2, S4) were used as controls (, each group). Real-time PCR results were reported as means ± error estimation of the calculated ratio using Taylor’s series, as appropriate for the DDCt method [39]. Specific activities values were given as means ± std. dev. The level of statistical significance was calculated by using factorial ANOVA and Holm-Sidak post hoc tests for multiple comparisons: ; .

(a)

(b)